av A Hansson · Citerat av 3 — af. Aberration coefficient of field curvature (1/m3) as. Aberration coefficient of spherical aberration (1/m3) diagnostic beam line lens at 488 nm wavelength as a function of the baffle aperture. high extinction ratio and large wavelength range.

Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies).

3,16. 0,35% 0,2. 0,8 microalga. 3. 83 112 101 192. 488.

Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0 AF 532 NHS AF532: Aldehyde C2 Aldehyde: Alexa Fluor 405 NHS AF405: Alexa Fluor 488 C5 Maleimide AF 488M: Alexa Fluor 488 NHS AF 488 Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes Extinction coefficients: AF488: ε dye = 73,000 cm-1 M-1 dsDNA: ε base = 6,600 cm-1 M-1 ssDNA: ε base = 8,900 cm-1 M-1 oligonucleotide: εbase = 10,000 cm-1 M-1 Example: A dye to base ratio of 0.05 corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 200 nucleotides or, respectively, into a 100 bp PCR Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). 4. Extinction coefficient behaviour with wavelength. The aim of this work is to study the atmospheric extinction coefficients at several wavelengths in different atmospheric conditions (clear and dusty days) over the Canarian observatories. The correlation between the coefficients provides information on the extinction wavelength dependency. 2002-09-01 2015-03-09 Extinction Coefficient (εmax) 7.3 x10 4 Fluorescence Quantum yield (Φf) 0 .92 Fluorescence Decay Time (τfl) 5.0 ns Brightness 67.2 Laser Line 488 nm Filter Set FITC Please Note: All products are for in vitro research use only and are not intended for use in humans or animals.

Extinction Coefficient: Length: _TEST1 AF 488: Alexa Fluor 532 NHS AF 532: Alexa Fluor 546 NHS AF 546: Alexa Fluor 555 NHS

Life. effects of instructed extinction. coefficient concept in psychological. Geijzendorffer, I.R. Cohen-Shacham, E., Cord, A.F., Cramer, W., Guerra, of succession and so far no non-indigenous species have resulted in the extinction of native species.

S6), Alexa Fluor 633-märkt Concanavalin A (a-d-glukos och a-d-mannos; ConA) to measure end-point fluorescence (excitation 488 nm/emission 520 nm). (JASCO V630 spectrophotometer) using an extinction coefficient of 12800 M −1

extinction due to other causes than merely habitat alterations. Estimation of the asymptomatic ratio of novel coronavirus infections (COVID-19). Het blijft belangrijk om beide af te nemen, omdat er patiënten zijn die alleen op één 56 778. 3,8 %. 150. [.

This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 is best suited to direct imaging of low-abundance targets. Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). Molar Extinction Coefficients vs. Absorbances for 1% Solutions Application of a molar extinction coefficient in the calculation yields an expression of concentration in terms of molarity: A / εmolar = molar concentration However, many sources, including the reference cited above, do not provide molar extinction coefficients. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.
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It is correlated to the extinction coefficient and a higher extinction coefficient gives a higher quantum yield. The chemical environment for example pH also affects the quantum yield. The molar extinction coefficient (ε) for Alexa Fluor 488 is 73,000 cm-1M-1.

Cathode-ray tutees,see p. 178 60 488.
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AFDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AFDye™ 488 is best suited to direct imaging of low-abundance targets.

Molar Extinction Coefficients vs. Absorbances for 1% Solutions Application of a molar extinction coefficient in the calculation yields an expression of concentration in terms of molarity: A / εmolar = molar concentration However, many sources, including the reference cited above, do not provide molar extinction coefficients. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.

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FluoProbes 488, 547H and 647H are found more photostable that is taken to good account in applications using long illuminations periods (i.e. scanning such as in confocal microscopy), or for longer shelf-life of reagents (i.e. manufacturing diagnostics).

better choice than Alexa Plus 647 which has higher QY and Extinction coefficient. "Alexa Fluor® 488 dye conjugate showed a 15–30% better signal- to-noise Alexa Fluor 488 excited at 492 nm and emitted at 519 nm and Alexa Fluor 555, extinction coefficient and fluorescence quantum yield (Janelia Fluor 549).122 absorption coefficient, quantum efficiency, photostability Extinction. Coefficient. (EC). [Mol−1cm−1].